Abstract
Objective: To determine the hepatoprotective effect of aqueous extract of Cichorium intybus (kasni) roots on
Pyrazinamide induced hepatotoxicity in male mice.
Study Design: Experimental randomized control study.
Place and Duration of Study: The study was conducted in the department of pharmacology at lslamic
International Medical College Trust (IIMCT), Rawalpindi in collaboration with Riphah Institute of
Pharmaceutical Sciences (RIPS), Islamabad and National Institute of Health (NIH), Islamabad. The duration of
th th
study was from 14 March, 2015 to 14 March, 2016.
Materials and Methods: The experimental randomized control study was carried out on fifty six male albino
Balb/C mice which were divided into four groups each having 14 mice. Group A (control) received normal diet
with no medication. Group B was given pyrazinamide in dose of 500mg/kg while group C and D were given
pyrazinamide along with low dose (200mg/kg) and high dose (400mg/kg) of Cichorium intybus roots (aqueous
extract) via gavage tube. At day 0, baseline blood samples were taken and normal values of serum alanine
aminotransferase (ALT) were obtained. Mid cycle samples were obtained to ensure the progress of study. Final
sampling was done at day 30 through cardiac puncture. Serum was separated in sterile tubes and biochemical
estimation was done on the same day. Mean and standard error of mean was calculated. Data was analyzed
through SPSS 20 and p value of <0.05 was meditated statistically significant.
Results: The extract of Chichorium intybus roots showed improvement in serum alanine aminotransferase
(ALT) values in group C and D. ALT value of 5-50UL was considered normal. Group A showed normal value of 24.5
UL. Group B showed severe hepatoxicity as indicated by the raised value of 143.5UL. The value of ALT in Group C
was 86.5 UL and it was 59.9UL in Group D.
Conclusion: Aqueous extract of Cichorium intybus roots has significant hepatoprotective activity in high doses
as compared to low doses.
Attiya Munir, Adnan Jehangir, Farhana Ayub. (2019) Hepatoprotective Effect of Aqueous Extract of Cichorium Intybus (Kasni) on Pyrazinamide Induced Hepatotoxicity in Male Mice, Journal of Islamic International Medical College, Volume-14, Issue-2.
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