Abstract
Amylases belong to one of the most important enzyme group with wide industrial applications. The amylase activity of 40 bacterial isolates was determined quantitatively. The effect of salt stress and a growth factor vitamin B1 (thiamine HCl) on amylase production potential was evaluated on two Bacillus spp., coded as BCTL-X-11 and BCTL-SL-101. In case of isolate BCTL-SL-101, 1% NaCl concentration produced highly significant increase in amylase activity as compared to 2 and 3% but BCTL-X-11 showed increased activity at 3% concentration after 48 hours incubation. Amylase production by BCTLSL-101 was maximum at 0.1% magnesium sulphate concentration but BCTL-X-11 showed maximum activity at 0.2% concentration after 48 hours incubation. The optimum concentration of dipotassium hydrogen phosphate for BCTL-SL-101 was found to be 0.5% but its optimum concentration for BCTL-X-11 was 1%. Vitamin B1 at 5.0 and 10.0 mg/l concentrations did not produce any rise in amylase activity up to 48 hours incubation, on the other hand highly significant inhibition was noticed at both concentrations.
SYED SHAHID ALI, MAHWISH NAZIR, TANZEELA RIAZ. (2010) EFFECT OF SALT STRESS AND VITAMIN B1 ON AMYLASE PRODUCTION POTENTIAL IN LOCALLY ISOLATED BACTERIA, Punjab University Journal of Zoology, Volume 25, Issue (1&2).
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