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Abstract
To determine the frequency of p53 and Ki-67 staining in cases of papillary urothelial carcinomas of urinary bladder and to correlate staining frequencies of p53 and ki 67 in low- and high-grade carcinomas. Study Design: A cross sectional study.Place and Duration of Study: The study was conducted in department of Histopathology, Foundation stthUniversity Medical College and Hospital Rawalpindi for duration of six months, from 1September 2017 to 28February 2018. Materials and Methods: Eighty-five patients of both genders undergoing trans urethral resection of bladder tumor were included in the study. The bladder biopsies were collected after TURBT and placed in 10% formal-saline for twenty-four hours. After tissue fixation, representative sections were taken and processed routinely for histology and immunohistochemistry at histopathology lab. Patients with histological diagnosis of low and high grade Papillary urothelial carcinoma were included in the study.Results: Among 85 patients, 64 were females and 21 were males. The most frequent tumor was low grade papillary urothelial carcinoma seen in 44 patients while 41 patients had high grade carcinoma. Positive p53 expression was more in high grade carcinoma (32.94%) as compared to low grade carcinoma (27.1%) and negative p53 expression was more in low grade carcinoma (23.5%) as compared to high grade carcinoma (14.1%). However, a statistically non-significant p value of 0.132 was obtained on comparison of p53 expression with chi square test. Similarly, positive Ki-67 expression was seen in 45.9% of low-grade carcinomas and 37.65% of high-grade carcinomas and negative expression was seen in 9.4% of low-grade carcinomas and 7.06% of high-grade carcinomas which did not correlate with our histopathological diagnosis. p value calculated by chi square test for Ki-67 was 0.659 which is also non-significant in our study.Conclusion: Our study concluded that p53 and Ki-67 immunostaining does not significantly correlate with histopathological grades of urothelial papillary carcinoma of urinary bladder in our local population. Therefore, immunohistochemistry expression of these markers cannot be solely relied upon for determining the grading of urothelial carcinomas.

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Dr. Iram Kehkashan Khurshid, Mumtaz Ahmad, Sarah Javed, Saba Aneeqa, Atiya Gardezi, Maryam Waqar. (2021) p53 and Ki-67 Expression in Papillary Urothelial Carcinomas of Urinary Bladder,An Experience at Foundation University Medical College Islamabad, Pakistan, , Volume 16, issue 2.
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Journal of Islamic International Medical College
Volume
Volume 16
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