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Objective: To determine the antioxidant effects of spinach on the weight of testes and epididymis in obese
Sprague Dawley rats.
Study Design: Experimental, randomized control study.
Place and Duration of Study: It was carried outfrom April 2016 to March 2017 in the department of physiology
of Islamic International Medical College, Rawalpindi, Pakistan.
Materials and Methods: Total 40 male Sprague Dawley rats of about 8 weeks were included. They were
randomly divided into Group A- Fed on standard diet and Experimental group- Fed on high fat diet to induce
th obesity. At the end of 6 week, after inducing obesity, experimental group was subdivided into Group B and
Group C. Weight of the testes and epididymis of Group A and Group B rats was recorded. Then 5% spinach was
given to Group C along with high fat diet for 4 weeks and finally weight of the testes and epididymis was
measured.
Results: Weight of the testes and epididymis (g) of Group B (1.32 ± 0.53g) rats was significantly decreased
(P<0.001) as compared to Group A (1.88 ± 0.92g) rats. However, weight of testes and epididymis (g) of Group C
(1.92 ± 0.49g) rats was significantly increased (P<0.001) as compared to Group B (1.32 ± 0.53g) rats after
spinach intake.
Conclusion: Intake of spinach supplemented diet has ameliorative effects on weight of testes and epididymis in
response to deleterious effects caused by obesity-induced oxidative stress.
Somia Iqbal, Shazia Ali, Arif Siddiqui. (2018) Antioxidant Effects of Spinach (Spinacia Oleracea) on Testicular and Epididymal Weight of Obese Sprague Dawley Rats, Journal of Islamic International Medical College, Volume-13, Issue-3.
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