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Genomic DNA isolation is fundamental step to study genetic diversity, phylogeny of species and cloning of desired genes. The
preferable protocol needs to be efficient, less time consuming, economic and non-destructive particularly for the endangered
species. The current study was aimed at economic isolation of considerable quantity of DNA manually from a particular tissue
of the fish without any potential damage to the animal. For this purpose fin tissue was preferably selected over scale due to its
universal occurrence in fish. A total of 180 samples of fin tissues belonging to twenty nine species of fresh water fish preserved
in different preservatives, were used for DNA isolation. DNA could not be isolated from the fin tissues preserved in formalin,
alcohol, air dried and ice preserved fins more than one week by any of two previously described methods including salt
extraction and urea treatment. However, introducing some modifications in salt extraction method resulted in large quantity of
high quality DNA from those fin tissues that were isolated from the living animal and immediately preserved in absolute
ethanol. The modifications include use of low quantity of proteinase K and slight increase in incubation period due to hard
nature of fin tissue. The extracted DNA by the modified salt extraction method was very pure, of high quality and resulted in
successful PCR amplification by Random Amplified Polymorphic DNA primer and cytochrome oxidase subunit 1 gene
specific primers. This modified procedure results in highest yield of isolated DNA reported so far. The method is particularly
suited for DNA isolation from endangered and sparse species as it requires minute quantity of fin tissue, without any
detrimental effect on fish.
Haji Muhammad, Zafar Iqbal, Muhammad Usman Iqbal, Tayyaba Younas, Qamar Bashir. (2016) An Efficient Method For Dna Isolation From Fish Fin, , Volume-53, Issue-4.
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