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Haemonchus contortus is a blood sucking gastrointestinal (GI) parasite that causes heavy production losses in small ruminants
due to their poor immune response and rapid development of anthelmentic resistance. Identifying genes involving in immune
related pathways against GI nematode infection will assist in better responding and curing the ill effects of infection with H.
contortus. Fecal egg counts and ear tissues were collected from 200 goats belonging to four breeds and PCR sequencing method
was used for identification of 25 seven single nucleotide polymorphisms (SNPs) and found two SNPs of dopamine receptor
binding 1 (DRB-1) (37 C/G; 10375 C/T), one from insulin-like growth factor1 (IGF-1) (58110 C/G), three from interleukin
(IL) 32 (7638 G/C; 9375 A/G; 1158 A/G) and one from IL-33 (70589 C/A) significantly (P<0.02) associated with fecal egg
count (FEC). Out of those seven SNPs, 5 were missense and changed the amino acids from (Pro Leu) in DRB1, IL32 (Arg Trp
and Ile Val) and in IL33 (Arg Trp). These missense SNPs may change the function of genes which play a crucial rule in
immunity of host and expulsion of GI nematode. Models of the SNPs of DRB-1, IL-32, IL-33 and IGF-1 genes were found
significant (P<0.02) indicating a strong association with FEC of H. contortous in goat. The haplotypes CCC and GCT of DRB1, AAG, AGG, GGC, GCA, and GGG of IL-32 gene were found significantly (P≤0.03) associated with FEC. The results of
linkage disequilibrium (LD) showed that most of the SNPs were in the range of (0.29-0.98). Therefore, the present study
investigated the role of these candidate gene SNPs in innate immunity and concluded that these SNPs were potentially
important for future goat breeding plans against H. contortus infection in goats.
Akhtar Rasool Asif, Sumayyah Qadri, Yuhua FU, MD. Abdul Alim, Zhenyang Wu, Nabeel Ijaz, Jianhua Cao. (2016) Single Nucleotide Polymorphisms In Drb1, Igf1 And Ils Associated With Fecal Egg Count Confers Resistance Against Haemonchus Contortus Infection In Goats, , Volume-53, Issue-4.
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