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In the present study a strain of Bacillus subtilis-BS06 was cultivated in submerged fermentation using agricultural wastes as substrates for carboxymethyl cellulase (CMCase) production under static and with agitation speed of 140 rpm. The whole fermentation experiments were carried out in 250ml Erlenmeyer flask. Different agricultural wastes such as sugarcane bagasse, wheat straw, rice husk, defatted soybean meal, corn cobs and wheat bran were exploited for CMCase production using different media (medium 1 (M1), medium II (M-II), medium III (M-III) &
medium IV (M-IV)).Of all these tested substrates, sugarcane bagasse was found to be the most suitable substrate for CMCase production. The effect of shaking on the production of CMCase demonstrated that sugarcane bagasse gave maximum CMCase activity (8.0 ± 0.32 IU) after 48h of fermentation period at 37oC with agitation speed of 140 rpm by using medium components III (M-III) and also medium M-IV. The highest enzyme production was observed in the following order; sugarcane bagasse > wheat straw > rice husk, wheat bran > soybean meal > corncobs. The crude CMCase enzyme was applied for saccharification of agricultural wastes and maximum saccharification (11.45%) was observed in bagasse. These results suggested its potential utilization in particular biofuel industry.
Mohsin Arshad, Muhammad Irfan, Asad-Ur-Rehman, Muhammad Nadeem, Zile Huma, Hafiz Abdullah Shakir, Quratulain Syed. (2017) Optimization of medium and substrate for CMCase production by Bacillus subtilis-BS06 in submerged fermentation and its applications in saccharification, Punjab University Journal of Zoology, Volume 32, Issue 2.
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