• Muhammad Furqan Shahid

• Tahir Yaqub

• Saima Yaqub

• Muzaffar Ali

• Muhammad Yasin Tipu

• Asim Aslam

• Azizul-Rahman

تلخیص

Poultry industry is confronting numerous viral infections particularly low pathogenic avian influenza virus (LPAIV) H9N2, which can bring ceptional abatement in trade of poultry products in endemic countries such as Pakistan. The current study was conducted to evaluate the infectious and antigenic potential of three different AIV (A/H9N2) variants isolated from commercial, backyard poultry and fancy birds (partridge). Commercial broiler birds were infected experimentally at the dose of 106 EID50 by using these three isolates. Results revealed that onset of clinical signs was started at 2nd day post infection (DPI) followed by severity in redness and swelling of eyes, sneezing and lacrimation at 3rd DPI that lasts up to 5th DPI. Cross reactivity of antiserum response toH9N2 was compared among reference isolates when challenged in broilers, Partridge origin anti-H9N2 antibodies showed varied hemagglutination inhibition results (4log2 to 15log2 ) when cross reacted with different antigens, suggesting H9N2 viruses are circulating with different antigenic characteristics. Sero-biochemical analysis of blood collected at 7th DPI revealed an increase in aspartate amino transaminase (246-308 U/L), alanine amino transferase (31-58 U/L) and blood urea nitrogen (11.21-22.58 mg/dl).

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