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The materials of water supply pipes exhibit differential tendencies to develop biofilms on the pipe surface. To evaluate the vulnerability of five types of commonly used pipe materials (HDPE, PC, PVC, PP and ABS) in water supply pipes, to biofilms formation, an annular reactor (AR) was operated. Twenty slides including 4 slides of each material were mounted in the AR. The source water (SW) bacteria were grown in TSB medium (100ml SW and 900ml TSB media) and were inoculated in the AR, followed by operation of the AR for 24 hours with the inoculum and 12 hours with continuous flow of SW. The genomic DNA was isolated from the biofilms formed on the slides, and amplicon libraries targeting V3 to V4 region of the 16S rRNA bacterial gene were prepared and sequenced using the Illumina MiSeq sequencer. Bioinformatic analysis with QIIME2 tools showed difference in bacterial composition in inlet water and biofilms formed on different pipe materials. At phylum level. Firmicutes (48 to 54%), Bacteroidetes (29 to 34%) and Proteobacteria (15 to 18 %) were the most abundant phyla in biofilms formed on different pipe materials. At genus level, Clostridium was the most abundant genus in biofilms of each plastic slide. In PICRUSt analysis biofilm formation pathways were mostly contributed by Aeromonas and Clostridium. Beside the deep insight into bacterial composition of biofilms, PVC pipe material was found the least prone to biofilm formation while the ABS material had the highest affinity to biofilm formation.
Junaid Ahmed Kori, Huma Tariq, Muhammad Raffae Vistro, Rasool Bux Mahar, Ishtiaq Ahmad Khan, Muhammad Shakee, Ramesh Goel. (2021) MOLECULAR EVALUATION OF BIOFILMS FORMED ON PIPES OF DIFFERENT MATERIALS DEPICTS CLOSTRIDIUM AND AEROMONAS SPP. DOMINATION, , Volume 18, Issue 3.
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