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In this work, we have isolated 66 phosphate solubilizing fungi from 75 rhizosphere soil of different medicinal plants around Malnad regions of Shivamogga district by serial dilution method using Pikovskaya’s media. Identification of fungal colonies was done by morphology and microscopic observation using standard manuals and they were labeled as PSF 1 to PSF 66. Fungi showed good results in siderophore production and phosphate solubilization under laboratory conditions. Out of 66 isolates, 56 PSF were positive for siderophore production and 10 PSF cultures showed negative for siderophore production. After screening, 10 PSF were selected for maximum siderophore production at 7 days incubation and their nature was detected and P solubilization was tested. In P solubilization, Solubilization Index - 1.08 to 3.96, colour change from blue to yellow on agar plate and red to yellow in broth, pH - 5.48 to 3.0 from initial pH of 6.89, Titrable acidity - 10.7g/L to 37.76g/L and phosphate in the culture broth - 290µg/mL to 20µg/mL and 10 PSF showed positive result for IAA production. Due to P solubilization capacity of the fungi and production plant growth promoters, they can be recommended as Phosphate bio-inoculums in agricultural field.
Venkataramanaiah Jyothi, Hirakannavar Veeranna Sowmya, Basaiah Thippeswamy. (2020) SIDEROPHORE PRODUCTION BY PHOSPHATE SOLUBILIZING FUNGI FROM RHIZOSPHERIC SOIL OF MEDICINAL PLANTS, , Volume 17, Issue 3.
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