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The main objective of this study was to find out mutations in the coding exon of GJB2 gene in the diabetic patients. Samples were collected from Lahore. A total of 50 type-Ⅱ diabetic patients were enrolled for this study. Blood samples were collected in EDTA vials. DNA was isolated from whole blood. PCR was done to amplify the exon of GJB2 gene. The PCR products were purified. Sequencing was done, in order to identify mutations in the coding region of GJB2 gene. The sequence analysis of PCR products i.e., the coding exon of GJB2 gene showed no mutations in the diabetic patients, to associate the gene with diabetes. Statistical analysis of these results also revealed no association of the GJB2 gene with diabetes.
Zainab Sharif, Nageen Hussain. (2020) MUTATIONAL ANALYSIS OF GJB2 GENE IN THE DIABETIC PATIENTS, , Volume 17, Issue 2.
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