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Cultural studies were performed to evaluate nutritional requirement, particularly C and N for effective growth of Alternaria. solani in PDA media. Out of eight different carbon sources tested, glucose gave was found the most effective for the growth of A. solani whereas poor growth was found in the control (without any carbon source). Among the seven nitrogen sources tested threonine followed by urea gave optimal growth of A. solani while minimum growth occurred in the control. Media pH at nine different values was investigated for the growth of A. solani. The media pH of 5-7 gave maximal growth whilst pH 9 revealed minimum. Evidently, A. solani requires acidic pH for its growth. A. solani required a temperature range of 25 to 30°C for maximal growth while minimum was obtained at 5 °C . Furthermore, alternate light and dark (12 h each) was found to be fairly effective for highest growth of A. solani. Morphological characters and growth were examined in eight different media. Colony characters included were: margin of colony, colour of colony and substrate colour while colony diameter represented the growth of A. solani. When tested for media, PDA gave the highest growth, corn meal agar also supported noteworthy growth of A. solani, whilst lactic acid resulted in poor growth.
Hina Zafar, S. Shahid Shaukat, Syeda Azra Tariq. (2019) A PHYSIOLOGICAL STUDY OF NUTRITIONAL AND CULTURAL MEDIA ON GROWTH OF ALTERNARIA SOLANI, THE CAUSES OF EARLY BLIGHT DISEASE OF TOMATO (LYCOPERSICON ESCULENTUM MILL.), , Volume 16, Issue 4.
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