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Cellulose, is water insoluble, tough & fibrous material that forms plant cell wall and the major constituent of the agro waste. Cellulose can be degraded into glucose by microorganisms through cellulase. This enzyme acts on the 14 glycosidic linkages in cellulose thereby releasing glucose. Cellulase production can be induced in certain microorganisms, such as bacteria and fungi, by culturing them on cellulose containing medium. The aim of present investigation was to optimize cellulase production in a newly isolated Bacillus sp. For this, the isolated Bacillus sp. was grown under varying physical conditions to explore the optimum temperature, pH and incubation time for maximum enzyme induction. The medium was supplemented with various carbon and nitrogen sources to further enhance enzyme production. It was found that optimal cellulase production was achieved at temperature of 37 °C, at pH 7.0 and after 48h of incubation. The best medium for cellulase induction contained wheat bran as carbon source and ammonium chloride used as nitrogen.
Madiha Sattar, Muhammad Noman Syed. (2019) OPTIMIZATION OF FERMENTATION CONDITIONS FOR THE BIO SYNTHESIS OF CELLULASE THROUGH NEWLY ISOLATED STRAIN OF BACILLUS, , Volume 16, Issue 3.
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