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Staphylococcus aureus has become a common nosocomial infectious agent throughout the world, causing a wide range of infections. Epidemiological studies suggest that hospitals of all sizes are facing the problem of the resistant form of Staphylococcus aureus that is Methicillin Resistant Staphylococcus aureus (MRSA). Protein A of Staphylococcus aureus is a virulence factor which is encoded by spa that shows a variation in length among different strains of MRSA. In this study, ninety-six strains of MRSA were collected from private and public sector hospitals of Lahore. Sixtyseven (70%) strains were confirmed as MRSA by DNase test and Kirby-Bauer disk diffusion test. The remaining (30%) isolates were misdiagnosed as other Staphylococcus species. Subsequently, polymorphic X region of the spa gene was amplified using specific primers of spa gene by the polymerase chain reaction (PCR). Four different banding patterns, ranging in length between 300 to 500 bp, showed polymorphism of the spa gene. Most strains showed 98 % homology with isolate spa 7 Staphylococcus aureus IgG protein. Those sequences can serve as a rapid diagnostic tool for the identification of local MRSA during outbreaks
Anam Tariq, Numan Javed. (2019) PREVALENCE OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) IN LAHORE, PAKISTAN ON THE BASIS OF STAPHLOCOCCAL PROTEIN A (SpA) TYPING, , Volume 16, Issue 2.
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