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To sustain life and promote good health, there is a need to provide sufficient amounts of safe and nutritious food as it is a basic human necessity. In the last few years, the risks associated with the food borne pathogens have greatly increased. The presence of Listeria monocytogenes in fresh and processed foods and listeriosis outbreaks has enforced the need of natural food preservatives. In this study, bacteriocin producing Lactobacillus plantarum KIBGE-IB45 was isolated from cheddar cheese. L. plantarum KIBGE-IB45 was identified on the basis of morphological, biochemical and 16S rDNA sequence analysis. The isolated strain was screened against several pathogenic strains for its bacteriocinogenecity. Results revealed that bacteriocin (BAC-IB45) possessed broad antibacterial potential against Listeria monocytogenes ATCC 7644 and other food borne pathogens. BAC-IB45 was partially purified using gradient precipitation method for further analysis. For the localization of bacteriocin coding gene, plasmid curing technique was performed which revealed that the bacteriocinogenic gene located on bacterial chromosome instead of plasmid. Broad antibacterial potential and antilisterial efficacy of BAC-IB45 suggests its plausible applications in food industry to overcome the unrestrained issues of chemical preservatives, food borne diseases, food quality, safety, shelf life and packaging.
Fariha Ibrahim, Afsheen Aman, Nadir Naveed Siddiqui, Raheela Rahmat Zohra, Shah Ali Ul Qader, Asma Ansari. (2019) SCREENING OF ANTILISTERIAL EFFICACY AND PARTIAL PURIFICATION OF CHROMOSOMALLY LOCATED BACTERIOCIN ISOLATED FROM LACTOBACILLUS PLANTARUM, , Volume 16, Issue 2.
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