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The antibacterial activities of different solvents (methanol, n-hexane, n-butanol, ethyl acetate and aqueous) extracted samples of Tamarix aphylla (L.) Karst. were checked against different microbes i.e Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Xanthomonas compestris and Klebsiella pneumonia using disc diffusion assay. Zone of inhibition (ZI) was calculated in percentage (%). In case of leaf samples, all extracts showed antibacterial activity against bacterial strains. The antimicrobial activity when the concentration of the extract was increased from 1 to 3 mg/disc. Aqueous phase, except for P. aeruginosa, X. compestris and E. coli at 3 mg/disc showed no activity against other microbes at all concentrations. The highest ZI was observed for ethyl acetate at 3 mg/disc concentration against X. compestris (86.0%) and P. aeruginosa (82.0%). In comparison to leaf extracts, the stem extracts showed comparatively high activities against all bacterial strains. The antimicrobial activity increased with increase in concentration (1-3 mg/disc). The aqueous phase except for B. subtilis at 3 mg/disc extract concentration showed no activity against other microbes at all concentration. In case of stem extracts, ethyl acetate showed maximum ZI (91.3%) against X. compestris and K. pneumonia (90.0%) at 3 mg/disc extract concentration. Higher concentration of phytochemicals i.e saponins, tannins, flavonoids, alkaloids and terpenoids were found in stem fractions as compared to leaf. The n-butanol and ethyl acetate fractions of both leaf and stem extracts showed high concentration of phytochemicals. The high antibacterial activities of n-butanol and ethyl acetate fractions may partly be attributed to the high concentrations of phytochemicals in these fractions.
Nida Jan. (2019) ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF DIFFERENT EXTRACTS OF TAMARIX APHYLLA (L.) KARST. (ATHEL TAMARISK), , Volume 16, Issue 2.
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