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This study was planned to investigate the anti-inflammatory, antibacterial and antifungal activity of chloroform, ethyl acetate, hexane and methanol extracts of leaves and stem of Physorrhynchus brahuicus against sixteen bacterial and eight fungal isolates. The anti-inflammatory activity of extracts was studied by analyzing the inhibition of albumin denaturation. The antibacterial and antifungal activity of different concentrations of extracts was analyzed by performing well-plate method. The methanol extract of leaves and stem showed maximum inhibition of albumin denaturation while chloroform extract of stem also showed inhibition but not considerable. In case of antifungal activity of leaves; the methanol, hexane and ethyl acetate extracts showed significant zones of inhibition compared with the zones of antibiotic nystatin. On the other hand; the hexane, chloroform and methanol extracts of stem showed considerable larger zones as compared to nystatin. These results justify that the inhibition by these extracts were similar and even more at various concentrations as the antibiotic nystatin showed. The largest zones of inhibition were observed at 5000ug concentration of approximately all the used leaves and stem extracts. In case of antibacterial activity, non-significant zones of inhibition were observed in all the extracts treatment. All the extracts of leaves and stem showed potential antifungal but very less antibacterial activity. These findings may support the use of Physorrhynchus brahuicus as an anti-inflammatory and antifungal traditional plant.
Sana Eijaz, Hira Ahmed, Aisha Sohail, Muneeba Khan, Anjum Parveen, Fouzia Haider. (2019) EVALUATION OF ANTI-INFLAMMATORY, ANTIFUNGAL AND ANTIBACTERIAL ACTIVITY OF PHYSORRHYNCHUS BRAHUICUS HOOK LEAVES AND STEM EXTRACTS, , Volume 16, Issue 2.
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