• Mehmood Khan

• Fozia Karam Khan

• Sadaf Moneeba

• Mehru Nisa

• Robina Manzoor

• Kaleem Imdad

• Kaleem Ullah

• Zahid Mehmood

• Ashif Sajijad

• Muhammad Ayub

• Noor Hassan

• Amir Rasool

تلخیص

Limonene is a transparent, colourless compound () has D and L isomer; which is found in oil of citrus fruits peels. It is a monoterpene (monocyclic) hydrocarbon and used in the production of several commodity chemicals, biodiesel products, medicinal compounds and pharmaceutical and it is also chemical an important precursor of many flavouring. Limonene is having low toxicity and can be used for several purposes. Overproduction of Limonene can be achieved by genetic engineering of Mevalonate pathway and 2-C-methyl-D-erythritol-4-phosphate pathway (MEP and MVA) in microorganisms by introducing limonene synthase. Limonene has several applications in industrial products and also can be used in clinical or medical field. Moreover the metabolic engineering of the pathways is potential production platform for any valuable limonene derivative. Limonene is flammable, function as biofuel. Limonene is naturally occurring in plants but genetically engineered microbes could produce sufficient quantity of limonene to cover the need but yet requires more efforts in labs to engineer more effective and active microbes for overproduction of limonene by developing Geranyl Pyrophosphate (GPP) pool in microbes.

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