• Bibi Zainab

• AsadUllah

• Zunera Tanveer

• Muhammad Umar

• Farah Sabeen Bugti

• Muhammad Naeem

• Taseer Ahmed Khan

• Azam Jan

• M. M. Tariq Kiani

• Saad Ullah Jan

• Ferhat Abbas

• Mohammad Zahid Mustafa

تلخیص

Rheumatoid arthritis (RA) is a systemic autoimmune disease that causes severe join problems. Adults suffering from RA are about 0.5-1% throughout the world. In serum, synovial fluid and other body fluids of RA positive patients, the presence of autoantibodies can be used as diagnostic biomarkers. Number of proteins has been expressed differently during the disease process against which the production of antibodies has been documented to date with diverse sensitivity and specificity. The study was designed to profile differently expressed proteins from sera of patients suffering from RA by SDS-PAGE. Separation of proteins was carried out by using SDS-PAGE and polyacrylamide gels were stained through Colloidal Coomassie Blue staining technique. Protein bands were visualized via transilluminator. Proteins expression of thirty five (male, n=11 and female, n=24) patient’s sera have been compared with twenty sera from healthy donors as control. Up regulation of 68 kDa protein was observed in 80 % sera, 50 kDa protein was up regulated in 77% sera of RA patients. Many bands of different molecular weights between 100 and 220 kDa have also been stained in patient’s sera but these bands are not documented to date.

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