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Twelve samples of peanut seed collected from different locations during 1992 were analysed for seed myrcofloral studies. Different species of Fusarium, Afacrophomina, Arthrobotrys, Aspergillus, Cephalosporium and Verticillium were recorded in different frequencies. In pathogenicity trial Fusarium equiseti, . macrophominaphaseolin, hisolani and i erticilliumalbo-atrum reduced germina-. tion over the control.
INTRODUCTION
Peanut (Arachis hk•ogeae L.) is an im-portant oil-seed crop commonly known as groundnut. It is a very good source of edible oil and is also consumed as dried fruit. It confers an area of 88900 hectares with an annual produc-tion of 96100 tonnes (Anonymous, 1991) in Pakistan. Peanut seeds are attacked by many seed borne fungi which cause serious losses to this crop (Richardson, 1979) and reduce germina-tion (Akhtar 1978. 1979 and Gilman, 1965). Present studies on peanut seed pathology report some more fungal flora and their effect on seed germination.
MATERIALS AND METHODS
Twelve samples of peanut seed were col-lected from local markets of Faisalabad, Sargodha and Khushab districts, during 1992 and were analysed for seed borne fungi in the Department of Plant Pathology. University of Agriculture. Faisalabad. Four hundred seeds from each satnple were plated by following standard blotter method (ISTA. 1976). Ten seeds were placed in each petridish having well
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moistened three layers of blotter paper and were incubated at 20 ± 2°C. for seven days. On eighth day petriplates were examined under stereornicroscope and fungi were identified un-der compound microscope with the help of available literature (Ellis, 1971. Booth, 1971) and maintained on plate dextrose agar medium (PDA) for pathogenicity trial. Four fungi viz. Alphaseolin, I ..a/bo-atrunt, F equiseti and Fsoiani, isolated from peanut seed, were checked in pathogenicity trial. Peanut seeds obtained from a healthy lot were treated with 5% ehlorox solution prior to their infesting with above mentioned fungi indi-vidually. Seeds were infested with 20 days old culture prepared in 200 nil distilled sterili
A. Shakoor Shakir, L. Muzzamal, Ashfaq Ahmad. (1994) In-vitro studies on seed pathology of peanut (Arachis hypogeae L.), , Volume 31, Issue 1.
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