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Karachi, the capital of Sindh is the largest city with the health issues caused by air and water pollution, improper sanitation and unhygienic measures. This study focuses on survey the human health issues of bacterial and viral pathogens in different age groups of the male and female patients. A total of 305 and 259 (total 564) different samples of stool and blood of the male and female patients of 1-15; 16-30, 31-45 and 46-60 age groups from Liyari (LY), Baldia Town (BT), Orangi Town (OT), Sachal Goth (SCG), Sohrab Goth (SBG) and Korangi Industrial Area (KIA) were collected to determine the occurrence (percentage) of major diseases prevailing in the environment of Karachi. In this study, the bacterial isolates were identified by culturing on Blood agar, MacConkey’s agar and Pylori agar for E.coli, Shigella dysentery and Helicobacter pylori respectively. HBVsAg method was used to detect the presence of antibodies in patients’ serum. Our results revealed the greater prevalence of E. coli / Shigella dysentery infection in females and males at SCG; Helicobacter pylori infection in at BT in males and no infection was observed in females and HBV infection resulted at greater ration in both genders at OT in 1-15 years of age respectfully. The observation of 16-30 years of age groups revealed greater occurrence of E. coli / Shigella dysentery SCG in males and in females at LY; Helicobacter pylori infection at LY in males and in females at KIA, whereas HBV infection at LY and KIA in males and females, respectively. The age groups 31-45 years showed greater incidence of E. coli / Shigella dysentery infection at LY and KIA; Helicobacter pylori infection at SCH and OT in females and males, whereas HBV resulted greatly at LY in males and in females at LY and OT, respectively. The findings of E. coli / Shigella dysentery; Helicobacter pylori infections revealed the greater incidence at LY, BT, LY, BT in males and females, respectively, whereas the HBV was observed at KIA in both genders of 46-60 years of age groups. These observations are the alarming increase in the risk factors and the dissemination of infections with greater morbidity and mortality rates in Karachi.

Wajahat Hussain Shah, Abdul Rasool Abbasi, Agha Asad Noor, Naeem Tariq Narejo. (2019) Frequency of Bacterial and Viral Infections in the Environment of Different Areas of Karachi, Pakistan Journal of Analytical & Environmental Chemistry, Volume 20, Issue 2.
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