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A rapid and reliable analytical method based on high-performance liquid chromatography (HPLC) with UV detection (221nm) has been developed for the determination of the anti-hyperglycemic agent Pioglitazone in pharmaceutical formulations and biological fluids (serum and urine) after clean-up with solid-phase extraction. Chromatographic separation was achieved with a Chromolithfi Performance RP-18e (1004.6mm) column using mobile phase composition of acetonitrile: mixed phosphate buffer (pH 2.5; 10mM) (30:70, v/v) with a flow rate of 2.0mL/min. The total run time was 2 min. under optimized conditions. The calibration curve was found to be linear in the range of 1-10 g mL-1 with regression coefficient of 0.9996, and the lower limit of detection 72 ng/20L injection. The method has been validated for the system suitability, linearity, precision and accuracy, limits of detection, specificity, stability and robustness. The %recovery of Pioglitazone in pharmaceutical formulations was found to be 104.7%. The assay has been applied successfully to the pharmaceutical Tablet samples and biological fluids (serum and urine) of healthy volunteers.
KamranAbro, NajmaMemon, M.I.Bhanger, S.A.Mahesar, ShahnazPerveen. (2011) Liquid Chromatographic Determination of Pioglitazone in Pharmaceuticals, Serum and Urine Samples, Pakistan Journal of Analytical & Environmental Chemistry, Volume 12 , Issue 1-2.
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