A simple, accurate and low cost spectrophotometric method for the determination of secnidazole in raw and pharmaceutical dosage forms has been developed. The proposed method is based on the reduction of the nitro group of the drug to amino group in the presence of Zinc dust and HCl. This reduced product then reacts immediately with p-benzoquinone and develops color, which has maximum absorbance at 532 nm. The calibration graph is linear over the concentration range of 12.5-160 µgml-1 with molar absorption coefficient of 1.5 × 103 Lmole-1cm-1.The common excipients and additives do not interfere in the determination. The proposed method is applied successfully to commercially available tablets and results are statistically compared with those obtained by reference method.
ATTA-UR-REHMAN, ABDUL SUBHAN EJAZ, ASAD RAZA, M.AFTAB SADIQ. (2004) Application of p-Benzoquinone to Spectrophotometric Determination of Secnidazole, Pakistan Journal of Analytical & Environmental Chemistry, Volume 5, Issue 2.