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The present study was designed to assess the presence/absence and
frequency of Barr Bodies on Polymorph Nuclear Neutrophils (PMNs) as a
confirmatory guide towards sex determination in cattle and buffalo. Blood
samples of adult cattle (n=26) and buffaloes (n=26) grouped as males
and females (n=13 each), were collected from the jugular vein through
appropriate measures. Thin blood smears were stained using Field stain
„A‟ and „B‟. The PMNs were counted and observed for Barr Bodies
attached to them. Only the neutrophils with terminable lobes were
examined and counted. The mean value ±SE of the Barr Bodies was
calculated and the difference between males and females of both species
was analyzed through independent T-test. The mean ±SE values for Barr
Bodies were significantly higher (P≤0.05) in female cattle (9.61±1.11) as
compared to their male counterparts (0.92±0.28). Similar results were
revealed for buffaloes being 6.23±1.15 and 0.92±0.28 for females and
males, respectively. In a nutshell, it is concluded that female cattle and
buffaloes have a higher occurrence of Barr Bodies on their PMNs as
compared to males. Furthermore, it can also be deduced that sex
determination in cattle and buffalo through this cyto-diagnostic technique
is quiet certain and precise.
MUSHTAQ HUSSAIN LASHARI, UMER FAROOQ, AMMARA ARSHAD, MUHAMMAD NAEEM TAHIR, MUSADIQ IDRIS, ANAM KHURSHID, ZIA UR REHMAN. (2019) Sex determination through Barr Bodies of Neutrophils in cattle and buffalo, Biologia – Journal of Biological Society of Pakistan, Volume 65 (I), Issue 1.
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