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The present study deals with laterosporulin biosynthesis from newly
isolated Brevibacillus sp. in liquid culture. Soil samples were collected
from different habitats of Lahore(Pakistan) for the isolation of bacterial
cultures. After primary and secondary screening, ISL-4 (0.53 IBU/ml) and
ISL-7 (0.6 IBU/ml) were selected having better potential for bacteriocin. A
significant (p≤0.05) increase in laterosporulin biosynthesis was observed
when the parameters like medium volume, buffer pH (7.0) and incubation
temperature (37°C) were optimized. Antimicrobial potential of
laterosporulin was also exploited using various techniques. Minimum
inhibitory concentration (MIC) and critical dilution assays (CDAs) were
performed for exhibiting bacteriocin inhibitory potential against various
indicator strains including Escherichia coli, Staphylococcus aureus,
Pseudomonas aeruginosa and Azotobacter sp. It was concluded that
Brevibacillus sp. possesses strong potential for laterosporulin
biosynthesis. Further manipulation of physical and nutritional parameters
improved its biosynthesis in liquid culture.
HANNANA MARYAM, SANA MAQSOOD , SIKANDER ALI. (2019) Laterosporulin Biosynthesis from a newly isolated Brevibacillus sp. in liquid culture, Biologia – Journal of Biological Society of Pakistan, Volume 65 (I), Issue 1.
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