Abstract
Livestock contribution to Pakistan’s GDP is 11.2% and it engages 8 million of rural families
for their livelihood. Estimated population of buffalo is 40.00 million heads in Pakistan mostly consisting of
low genetic worth population resulting in low productivity and pressure on natural resources. Assessment
of genetic diversity and genetic characterization of indigenous livestock resources is an essential step towards
conservation and to gauge effects of various breed improvement efforts on population genetics.
Methods: In present study genetic diversity of Nili Ravi buffalo population was assessed. A total of 196 unrelated
Nili Ravi buffaloes from Punjab, Pakistan were sampled in this study. Genomic DNA was extracted and subjected
to amplification using FAO recommended 12 Short Tandem Repeats (STRs) microsatellite markers. Among 12
microsatellite loci, 11 were successfully amplified (TGLA227, BM2113, ETH10, SPS115, TGLA126, TGLA122,
INRA23, BM1818, ETH3, ETH225 and BM1824) whereas microsatellite locus TGLA53 was not amplified.
Amplicons were resolved by genetic analyzer instrument and gene mapper software. Allele count, frequencies,
gene diversity, heterozygosity, polymorphic information content (PIC) and linkage disequilibrium values were
calculated by using Microsatellite toolkit v3 and Power Marker version 3.25.
Results: A total of 96 alleles were detected in 196 samples with average of 8.73 alleles per locus and range of
5 alleles (ETH 3) to 18 alleles (ETH 225) per locus. Gene diversity ranged from 0.198 (BM1824) to 0.841
(ETH225), observed heterozygosity values ranged from 0.081 (ETH10) to 0.831 (BM2113) and PIC values
ranged from 0.191 (BM1824) to 0.825 (ETH225).
Atia Bukhari, Naveed Ahmed, Fizza Khan, Muhammad Shafique, Asif Suleman Sahi. (2019) Assessment of genetic diversity and genetic characterization of Nili Ravi buffalo breed utilizing microsatellite markers, Advancements in Life Sciences, Volume 7, Issue 4.
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