Abstract
Pseudomonas aeruginosa (Ps. aeruginosa) is considered as an opportunistic pathogen and
the leading cause of morbidity and mortality in immunocompromised individuals. Globally, approximately
10-15% of the nosocomial infections are caused by Ps. aeruginosa. The Ps. aeruginosa can acquire
resistance against broad-spectrum antibiotics. According to recent studies increased mortality has been
observed due to infection with extended-spectrum-beta-lactamase (ESBL) producing Ps. aeruginosa strains.
This study was designed to determined antibiogram of ESBL producing multi-drug resistant Ps. aeruginosa in
Khyber Pakhtunkhwa.
Methods: The clinical confirmed Ps. aeruginosa samples were collected according to the standard protocol, at
Khyber Teaching Hospital (KTH), Peshawar. All collected samples were sub- cultured on appropriate culture
media. After isolation and identification, the antibiotics susceptibility testing was performed. The detection of
ESBL was carried out by the double-disc diffusion method. Carbapenemase-producing bacteria was confirmed
by the modified Hodge test. Descriptive analysis was performed for statistical analysis of collected data.
Results: A total of one hundred and sixty-two (n=162) Ps. aeruginosa confirmed isolates were collected, in which
59.3% were male and 40.7% were from female patients. The percentages of ESBL and carbapenemase
producing Ps. aeruginosa isolates were 5.5% and 23.5%, respectively. The multidrug resistance was observed
against 27.2% isolates. Among tested antibiotics highest percentages of resistance was observed against
ciprofloxacin (43%) and ceftazidime (39.5%).
Conclusion: We observed highest level of drug resistance in Ps. aeruginosa clinical isolates against tested
antibiotics and majority of the isolates were Multi-drug resistant (MDR).
Anees Muhammad, Ihsan Ali, Nasir Ali, Muhammad Owais, Sadiq Noor Khan, Irfan Qadir Afridi. (2019) Evaluation of Antibiotics Pattern of Extended Spectrum BetaLactamase Producing Multi-Drug Resistant Pseudomonas aeruginosa, Advancements in Life Sciences, Volume 7, Issue 3.
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