Abstract
Gradual increase of antibiotic resistance is a global problem. In this study, we have
developed an alternative approach as an alternative to conventional antibiotics from the
natural source to solve the antibiotic resistance problem. Some normal microflora were
isolated from healthy human skin, their antimicrobial efficacy were examined against
some skin and intestinal pathogens initially by cross streak method and finally by disc
and well diffusion method. Two normal microflora (e.g., Bacillus licheniformis and
Corynebacterium jeikeium) were observed producing antimicrobial metabolites which
were effective against Klebsiella pneumoniae subsp. pneumoniae, with maximum
antimicrobial activity at 250C, 48h, pH 9 and 370C, 72h, pH 7 respectively. Only the
antimicrobial metabolites produced by Bacillus licheniformis was detected as
bacteriocin like substances which was further confirmed as antimicrobial peptide
through papain treatment. Efficacy of crude bacteriocin like substances was compared
with 10 commercially available antibiotics against Klebsiella pneumoniae subsp.
pneumoniae. Of these, 4 antibiotics were found resistant but crude bacteriocin like
substances along with 6 other antibiotics showed remarkable susceptibility. Therefore,
more studies on the efficacy of this bacteriocin like substances needs to be done to fully
understand its mechanism and potentiality as novel antimicrobials.
Reazul Karim , Nuruddin Mahmud, Mohammad Abdul Hakim. (2019) Detection of bacteriocin like substances from normal skin microflora as alternative to conventional antibiotics, Asian Journal of Agriculture and Biology, Volume 7, Issue 4.
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