Abstract
The study characterized the lactoferrin (Lf) gene in different water buffalo breeds and
determined its association with subclinical mastitis (SCM). A total of seventy five (75)
and five (5) milk and blood samples, respectively were collected for the conduct of this
study. Amplified Lf gene demonstrated a 2224 base pairs (bp) molecular weight.
Nucleotide and amino acid sequence of Lf gene of riverine- and swamp-type water
buffaloes revealed 98.83% and 98.29% identity, respectively with that of Bubalus
bubalis Lf gene in the GenBank. Phylogenetic studies showed that Lf genes of both
types of water buffaloes grouped with Lf gene of water buffalo sequences registered in
the GenBank. Three water buffalo genotypes were documented using the restriction
enzymes AluI and HaeIII. The AluI can produce three genotypes (AA, AB, BB)
resulting in three cuts of 561, 217 and 123 bp. Using HaeIII, three restriction patterns
were observed producing three genotypes as well with four fragment sizes of 561, 318,
112, and 70 bp. Based on the statistical analysis, no direct association has been
established between the Lf genes of water buffalo with SCM using AluI and HaeIII
restriction enzymes although a higher number of animals with genotype BB belongs to
those with SCM group. The results of the study merit for further studies regarding the
BB genotype of Lf gene as a possible resistance gene marker for SCM in water
buffaloes.
Yancy Ogdamin Waminal, Gabriel Alexis San Perdo Tubalina, Claro Niegos Mingala. (2019) Molecular characterization of lactoferrin gene as genetic marker to subclinical mastitis in water buffaloes (Bubalus bubalis), Asian Journal of Agriculture and Biology, Volume 7, Issue 4.
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