Abstract
Alternaria fruit rot (AFR) caused by Alternaria alternata is a serious threat to grape
market valuesin Pakistan during post-harveststages, at the time of handling, storage
transportation and distribution respectively. The objective of this study is to
determine the anti-fungal activities of plant essential oils against Alternaria
alternata causing fruit rot of grapes. For this purpose, three selected plant essential
oils (EOs) viz. Kikar (Acacia karoo), Moringa (Moringa oleifera) and Sukh chain
(Pongamia pinnata) essential oils at 200, 400 and 600 ppm concentrations were
investigated by using different methods under in vitro condition against previously
isolated culture of A. alternata designated as (Isolate ID. AA4PL1) on grape
bunches. Results showed that Plant EO of Sukh chain at all concentrations showed
significantresult to inhibit themycelial growth (89.4, 92 and 96.2 percent) in contact
assay method as well as 96.2, 97 and 98.2 % growth inhibition regarding fungal
culture transfer(FCT) experiment while, in case of well diffusion method 32%, 41%
and 48% growth inhibition was recorded at 7
th day of incubation followed by
Moringa EO and Kikar EO as compared to control that showed 0 % growth inhibition
was measured. Moreover, results related to spore germination assay revealed that
Sukh chain essential oil at 200, 400 and 600 ppm showed significant inhibition of
germ tube length of A. alternata (140.8 µm, 77.5 µm and 34.1 µm) as compared to
control in which germ tube length was recorded 250 µm respectively. It was
concluded that Sukh chain EO has a great potential to inhibit the growth of A.
alternata and can be further used as a strong antifungal agent against this pathogen
under in vivo condition.
Awais Sajid, Gulshan Irshad, Farah Naz, Salman Ghuffar, Imran Hassan, Nasir Mahmood, Komal Rani, Muhammad Faizan Manzoor, Ali Meesam, Abdul Mannan Hamzah, Muhammad Zunair Karamt. (2020) In vitro evaluation of plant essential oils against Alternaria alternata causing fruit rot of grapes, Asian Journal of Agriculture and Biology, Volume 8, Issue 2.
-
Views
760 -
Downloads
68
Article Details
Volume
Issue
Type
Language
