Abstract
Objective: To analyze the resistance patterns of antibiotics against infectious agents causing blood, urine and pus infections.
Study Design: Prospective experimental study.
Place and Duration: Pharmaceutical Microbiology Lab, Department of Pharmaceutics, Faculty of Pharmacy, Hamdard University,
Karachi from 8th January to 21st June 2018.
Methodology: As 126 Clinical isolates of E.coli and Klebsiella spp. were collected from various pathological laboratories of Karachi.
Antimicrobial susceptibility testing was performed by Kirby-Bauer method for disks of four antibiotics; Imipenem, Cefotaxime,
Nalidixic acid and Gentamicin.
Results: Among 126 clinical isolates, (66%) are Escherichia coli, (34%) are Klebsiella species. (63%) isolates have been obtained from
the urine culture, (33%) from blood and (4%) from pus causing urinary tract infection, bacteremia and soft tissue infection
respectively. Imipenem is found to have significantly (p=0.0001) highest susceptibility against E.coli (87%) and Klebsiella
specie.(91%). However, E.coli (40%) and Klebsiella specie. (93%) are highly resistant from Cefotaxime, while almost 50% organism
are resistant from Gentamicin (53%) and Nalidixic acid (33%).
Conclusion: Imipenem has been found to be the most effective of all tested antibiotics while Cefotaxime has developed resistance
from these microorganisms.
