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Abstract
Sugarcane is a complex allopolyploid hybrid with erratic flowering due to various geo-climatic factors hence deliberate hybridization for varietal improvement is a dilemma in most of the countries where sugarcane is cultivated. Somaclonal variations is an alternate strategy used for development of disease resistance and improvement in yield attributes, but point mutation in candidate genes is questionable. The aim of this study was to develop somaclones resistant to red rot and Sugarcane mosaic virus (SCMV) in obsolete sugarcane varieties and their genetic integrity assessment in candidate gene’s exon regions. A total of 581 somaclones were developed, of which 201 survived after hardening and 121 after transplantation and screened against red rot and SCMV. Only 10% somaclones were found resistant to red rot in susceptible and moderately susceptible varieties while SCMV concentration was recorded less than negative control in somaclones. Four candidate genes namely; catalase (CAT1), sucrose phosphate synthase (SPS), gibberellin 2-xidase 4 (GA 2-oxidase 4) and tillering branched 1 (TB1) were evaluated for possible SNPs in their exon regions. Sequences of five representative somaclones were aligned with their parental clones and no possible SNP changes were observed. Somaclonal variations was a good source of genetic improvement of sugarcane for disease resistance with no SNP changes in candidate genes.

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Muhammad Shahzad Ahmed, Dilnawaz Ahmed Gardezi, Jacqueline Batley, Satomi Hayashi, Manuel Zander, Aslam Javid, Muhammad Zaffar Iqbal, Shahid Iqbal Awan. (2019) SOMACLONAL VARIATIONS FOR RED ROT AND SUGARCANE MOSAIC VIRUS RESISTANCE AND CANDIDATE GENES INTEGRITY ASSESSMENT IN SOMACLONES OF SELECTED SUGARCANE VARIETIES (Saccharum officinarum L.), , Volume 56, Issue 1.
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Pakistan Journal of Agricultural Sciences
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Volume 56
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