• Sohail Kamaran

• Tariq Manzoor Khan

• Ali Bakhsh

• Nisar Hussain

• Shahzadi Mahpara

• Abdul Manan

• Waqas Shafqat Chattha

• Tehseen Ali Jilani

• Javaria Sherani

• Mujahid Iqbal

Abstract

Cotton is an essential fiber and oilseed crop cultivated in semi-dry to arid atmosphere rage in various parts of the world. For a successful breeding program, it is of extreme significance for the plant breeders to have knowledge of genetic variability present in the breeding material. Therefore, present investigations were carried out to make sense of genetic relatedness and differences on the basis of morphological traits (tap root length, lateral root number and root volume) and molecular markers (SSR) among 30 upland Bt cotton genotypes developed by public and private sector. Results of morphological data indicated that maximum Tap root length, lateral root numbers and root volume were recorded for genotypes BH-178, BH-180 and CIM602 respectively. So, these genotypes may be used in breeding program to improve respective trait. Meanwhile for molecular study Out of 40 markers, 20 were found polymorphic among 30 genotypes. Total number of alleles opened up by these markers were 116 while 90 were found polymorphic demonstrating 22% genetic diversity with 4.50 alleles for each locus on an average. Dendrogram demonstrated a most extreme range of closeness from 78 to 98%. Least likeness (78%) was seen among the lines FH-142 and TARZAN-1, though, most extreme (98 %) was seen between the lines FH-113 and FH-114. It is direly expected to incorporate exotic cotton germplasm to expand magnitude of genetic variability which may bring improvement in seed cotton yield and other economic traits in cotton crop.

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