Abstract
Peanut (Arachis hypogeae Linn.). zygotic embryos (ZE), plumular apices (PA) and embryonic Axis (EA) of cv. NC-7 were
pretreated for 15 days on Murashige and Skoog (MS) medium enriched with 25 mg.L-1 6-Benzylaminopurine (BA).
Pretreated explants were then post treated on semi solid agar gelled MS medium possesses BA concentrations ranged 0.25 to
3.0 mg.L-1 for a period of 8 weeks. Callus and shoot induction was registered on all explants irrespective of BA pre-and post
treatments. The highest shoot counts per explant ranged 2.67-4.43 (ZE), 2.33-4.11 (PA) and 1.87-3.33 (EA). Average shoot
length ranged 1.75-3.62 cm (ZE), 1.45-3.70 cm (PA) and 2.10-2.60 cm (EA). Successful rooting (100%) of shoots
regenerated were achieved on agar gelled MS medium provided with Indole-3-butyric acid (IBA) at the rate of 1.0 mg.L-1
.
Successful adaptation of rooted plantlets were done by transferring them to pots filled with vermiculite. This study
highlighted the high potential of micropropagation of three different explants in peanut biotechnology.
