Abstract
Cotton leaf curl virus (ClCuV) has the most damaging and rapidly evolving virus spp infecting cotton (Gossypium hirsutum
L.) and make cotton leaf curl disease complex. CLCuD is caused by Bemicia tabaci transmitted monopartite begomovirus
whose genome is composed of DNA-A and satellite components. Present study of cotton was designed to amplify the circular
single stranded molecule of begomovirus associated with CLCuD through RCA technique. Twenty lines/varieties of cotton
were evaluated for their response against cotton leaf curl virus. Total genomic DNA from these samples were isolated from
cotton varieties/lines and full length viral DNA-A components from CLCuV infected cotton plants were polymerized through
PCR by using universal begomo 01 and begomo 02 primers. Further insilico study of different viruses with restriction enzymes
PstI and EcoRI was done. The residual viral titer which was evaluated through PCR, its value was compared with the visually
observed symptoms to evaluate the plant response. All varieties showed disease symptoms of which susceptible varieties were
FH-114. MNH-886, LALAZAR, MS-71, MS-289, FH-682, FH-900, Z-31, ALI AKBR-703, N-112. Moreover, moderate
susceptible varieties were FH-942, FH-142, MNH-86, CM-595, HUMA-15, ALI AKBR-905. However, moderate resistant
varieties were FH-941, CM-595, ALI AKBR-905, CA-12.
