Abstract
Objective: To highlight the prevalence and epidemiology of New Delhi metallo-ß-lactamase-1. producers in pus
samples.
Methods: The cross-sectional study was conducted from April to August 2018 at the Biotechnology Laboratory,
Balochistan University of Information Technology Engineering and Management Sciences, Quetta, Hi-tech
Laboratory, Centre for Advance Studies in Vaccinology and Biotechnology, University of Balochistan, Quetta and
Microbiology Laboratory, Bolan Medical Complex Hospital, Quetta, Pakistan. Biochemical and molecular
approaches were used for the identification of the isolates and Modified Hodge Test for the phenotypic detection
of class-A carbapenemase activity. Minimum Inhibitory Concentration was performed using E-test and broth
microdilution method. Molecular basis of carbapenemase activity was ascertained by the recognition of blaNDM-1
gene in the isolates.
Results: Of the 300 pus samples taken from surgical/burn units, 6(2%) blaNDM-1 harbouring isolates were found;
3(50%) each being Klebsiella pneumoniae and Pseudomonas aeruginosa. Klebsiella. pneumoniae isolates were
extensively drug-resistant. The Pseudomonas aeruginosa isolates displayed resistance against 21 antibiotics of
tetracyclines, quinolones, β-lactams, aminoglycosides, monobactams, sulphonamides, macrolides, cephalosporins,
phosphonic acid and polypeptide groups, suggesting pan-drug resistance.
Conclusion: The resistance pattern of the bacterial isolates poses a significant clinical threat in the region.
Mohammad Din, Khan Muhammad Babar, Dawood Shah, Ghulam Sarwar, Saleem Khan, Hummaira Sadaf, Shahjahan Shabbir Ahmed, Faiza Liaqat Ali, Muhammad Yasir, Nazeer Ahmed. (2021) Dissemination of NDM-1 in pseudomonas aeruginosa and klebsiella pneumoniae isolated from pus samples in tertiary care hospitals of Quetta, Pakistan , Journal of the Pakistan Medical Association, Volume 71, Issue 1.
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