Abstract
This descriptive cross-sectional study was performed in
the Department of Microbiology, Fauji Foundation
Hospital, Rawalpindi, from March 2019 to September
2019 to determine the in vitro efficacy of Daptomycin
against clinical isolates of Methicillin-Resistant
Staphylococcus aureus (MRSA). Consecutive nonprobability sampling technique was used and a total
number of 270 patients' Pan Cultures having MRSA
growth on Cefoxatin Disc with size less than 22 mm zone
size were included in the study. Cultures were inoculated
on MacConkey, Chocolate and Blood agar and then
incubated for 24 hours at 37 degree Celsius. After
incubation, Coagulase test, Catalase test and Gram
staining technique were used for further identification.
Minimum Inhibitory Concentration (MIC) of the isolates
for Daptomycin was obtained by using E strips (Oxoid UK)
according to Clinical & Laboratory Standards Institute
(CLSI) guidelines. The mean age of the patients was
46.73±12.22 years, and the study included 147 (54.44%)
males and 123 (45.56%) females. Regarding the type of
specimen, there were 154 (57.04%) pus specimens, 54
(20.00%) blood specimens, 27 (10.00%) fluid specimens,
18 (6.67%) urine specimens, 10 (3.70%) high vaginal
swabs (HVS) specimens and 7 (2.59%) sputum specimens.
Daptomycin was effective in 264 (97.78%) patients with
MIC range from .015 to 1 μg/ml on E strip.
Asim Ali Shah, Shahid Ahmad Abbasi, Yasir Ali, Ayesha Maqbool. (2021) In vitro efficacy of Daptomycin against clinical isolates of Methicillin-resistant Staphylococcus aureus (MRSA) , Journal of the Pakistan Medical Association, Volume 71, Issue 1.
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