Abstract
Onion seedlings with wilt symptoms were collected from the state of Guerrero, Mexico, from which Fusarium oxysporum f.
sp. cepae was isolated and identified, based on the colony and conidia structure´s morphological characteristics. A
pathogenicity test was conducted, inoculating healthy onion seedlings with the isolated pathogen, while the control seedlings
were sprayed with sterile distilled water only. Control seedlings remained healthy while inoculated ones developed wilt
symptoms seven days after inoculation, similar to those observed in the production area. F. oxysporum f. sp. cepae was reisolated and the genomic DNA extraction, PCR, and sequencing test were carried out. Based on morphological identification
and pathogenicity tests, it was determined that F. oxysporum f. sp. cepae was the causal agent of wilt in onion, although the
molecular identification was only carried out on a genus level. Additionally, different biocontrol products were evaluated in
vitro including fungicides and phyto-extracts in order to determine the inhibition of the pathogen. The products used were as
follows: Trichoderma asperellum strain obtained at CSAEGro (100%), T. harzianum (100%), prozycar (100%), cupravit
(100%) manzate (100%), busan (100%), Q-2000 (60%), neem extract (51%) and garlic extract (27%)
