Abstract
Objective: The objective of this study was to observe changes in spermatogenesis testes of albino rats exposed
to intraperitoneal lead acetate and to look for the reversibility of these changes after cessation of lead acetate and
subsequent oral administration of honey.
Study Design: Experimental animal study.
Place and Duration of Study: National Institute of Health Islamabad from January to June, 2009.
Materials and Methods: Animals were obtained from the animal house of N.I.H and were divided into three
groups A, B and C. Group A was subdivided into two groups A-I and A-II. Group B was also subdivided into two
sub groups; B-I & B-II. Group C was not subdivided into subgroups. The animals in groupAwere used as control,
while those of groups B and C were treated with lead acetate that was given intraperitonially in the dose of
4mg/kg body weight, 5 days a week for 6 weeks. The animals in group B-I were sacrificed at the end of six week to
observe the toxic changes while animas in group B-II were kept alive for another 6 weeks on normal diet. The
animals in group C were given honey in dosage of 10ml/100ml water with normal diet for further 6weeks. These
groups (B-II and C) were then sacrificed after 12 weeks to observe the effects of honey on spermatogenesis.
Results: The histological comparison of testes of both groups of animals showed that after six weeks, the width
of germinal epithelium and the number of spermatogenic cells had decreased in lead toxic groups as compared
to the control rats (p<0.05) and in majority of the seminiferous tubules, the basement membrane was disrupted.
The width of germinal epithelium, and the number of spermatogenic cells were improved after oral administration
of honey.
Conclusion: This study provides evidence that lead has toxic effects on testis which are partially reversible on
oral intake of honey.
Saffia Shaukat, Imran Qureshi, Rehmah Sarfraz. (2011) Effects of Honey on Lead Induced Changes in Spermatogenesis, Journal of Islamic International Medical College, Volume-6, Issue-2.
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