ELISA is a serological assay claimed for higher sensitivity and specificity in diagnosing sub-clinical fascioliasis. The
purpose of this investigation was to evaluate the sensitivity and specificity of an ELISA kit (DRG®, Germany) for the
diagnosis of small ruminant fascioliasis in district Sargodha, Punjab, Pakistan. Microscopically, 226 animals out of 600
(37.66%) were found positive for fascioliasis. Higher prevalence (38.78%; 121/312) was found in goats as compared to
sheep (36.46%; 105/288). Prevalence of Fasciola hepatica was significantly higher as compared to Fasciola gigantica
(p<0.005). Commercial ELISA kit detected anti-fasciola antibodies in higher percentage in sheep and goat population as
compared to coprological examination. Seroprevalence was 48.61 and 49.36% in sheep and goat population. Commercial
ELISA kit was more specific than coprological examination when compared in Win episcope by screening of comparative
diagnostics.
Rubina Anjum, Muhammad Nisar Khan, Muhammad Sohail Sajid, Muhammad Tariq Javed. (2015) Evaluation Of Commercial Elisa Kit For Diagnosis Of Small Ruminant Fascioliasis In Pakistan, , Volume-52, Issue-1.
