Abstract
A tomato male sterile line TMS1, tolerant to cucumber mosaic virus (CMV) was crossed with 5 high yielding but CMV
susceptible lines namely Naqeeb, Money Maker, B23, B25 and Peto-86 to transfer the resistance into F1 hybrids. The entire
breeding material was subjected to mechanical inoculation followed by double antibody sandwich procedure (DAS-ELISA)
to confirm the presence or absence of CMV. Data was recorded on the percentage of disease transmission, mean latent
period, average disease severity and yield and yield related traits in field. TMS1 was reconfirmed ‘tolerant’ through
mechanical inoculation but ‘resistant’ in natural filed conditions. Hybrids did not express tolerance level at par with TMS1 in
mechanical evaluation. However, they showed at par response under field evaluation except hybrid TMS1 × B25. Hybrids
TMS1 × Naqeeb, TMS1 × MM, TMS1 × B23, TMS1 × B25 and TMS1 × Peto-86 showed better tolerance to CMV over
standard hybrids T-1359 and Salar. Line TMS1 proved to be a valuable donor of CMV resistance in cultivated tomato.
