Abstract
Chalcone synthase gene is a key enzyme in the biosynthesis of flavonoids and isoflavonoids. This gene assumes several
functions in plants such as protection against UV ray and defense against pathogenic factors. In this study, chalcone synthase
gene was studied and evaluated in terms of evolutionary rate in 119 species of creatures such as agricultural plants as eukaryote
creatures and bacteria as prokaryotes. The evolutionary analyses were performed using bioinformatics tools. After preparing
the phylogenic tree, the evolutionary study and comparison were performed. Also divergent time of chalcone synthase gene
was investigated in the agricultural plants and bacteria. This study led to obtaining two general conclusions: First, the
evolutionary rate between two prokaryote and eukaryote groups is different over time, and second, considering the calculation
of divergent time for the genome of prokaryotes from eukaryotes in the previous works, it is recommended to consider the
Chalcone synthase gene duplication time as about 410 million years.
