Abstract
Cotton, referred as “The white gold” is an important commercial crop in India and stands third in the world by means of area
of cultivation. Cotton plant regeneration from callus by somatic embryogenesis and its efficiency has been improved
significantly in recent times. Our primary investigation was on regenerative studies and multiple shoot induction system
focusing mainly on meristematic tissues like seedling cotyledonary nodal explants in RAH-9750 cotton cultivar. An attempt
has been made to improve the rate of surveillance and growth of regenerated cotton seedlings by bio-inoculant (mainly AMF)
treatment under greenhouse conditions. Out of a total seven pure cultures of AMF fungi, R1-R2 have shown maximum
mycorrhizal colonization with RAH-9750 (R) and was identified as Glomus mosseae. This variety was also tested with three
different bioinoculants i.e., Rhizobium sp. RHPU-7, Azospirillum sp. PPK-27, Bacillus sp. PU-1, apart from AMF R1-R2 in
different combinations. The cotton seedlings have shown the best results in single, dual, triple and multiple combinations i.e
R+R1-R2, R+R1-R2+Rhizobium, R+R1-R2+Rhizobium+Azospirillum and R+R1-R2+Rhizobium+Azospirillum+Bacillus
respectively. The growth of cotton plants (RAH-9750) generated from meristematic tissue culture was found to be increasing
significantly when compared with the normal seeds. Similar results were noticed when the same experiment was subjected to
the different soil types of Mahabubnagar district. The investigation clearly infers that better yield of cotton RAH-9750 (R)
could be achieved by treating the regenerated cotton seedlings with bioinoculants in different combination in various soil
types of Mahabubnagar district.
