Home Journal List Pakistan Journal of Agricultural Sciences Volume-51 Article Details

Abstract
The research work was aimed for isolation and identification of Aspergillus flavus from poultry feed samples using conventional and molecular techniques. Out of 23 samples, 8 (34.78%) were found positive for A. flavus. The molecular techniques comprise comparison of ITS1-5.8S-ITS2 region. In addition, the ability of the isolates to express aflatoxin synthase (CYP64A1) gene in synthetic liquid medium was also analyzed. Based on cultural characteristics, unique nature of the ITS 1 and 2 genes and expression of CYP64A1 at mRNA level, A. flavus was accurately identified from the feed samples. The isolated A. flavus would be a potential candidate for further studies and application.

Keywords

K.H.M. Nazmul Hussain Nazir, Jayedul Hassan, Pradeepraj Durairaj, Hyungdon Yun. (2014) Isolation And Identification Of Aspergillus Flavus From Poultry Feed Samples Using Combined Traditional-molecular Approach And Expression Of Cyp64A1 At mRNA Level, , Volume-51, Issue-2.
  • Views 295
  • Downloads
Previous Article 
Next Article  

Article Details

Journal
Pakistan Journal of Agricultural Sciences
Volume
Volume-51
Issue
Type
Language


Recent Volumes