Abstract
A Gram-stain negative, cocci to short rod, non-motile, strictly aerobic, heavy metal-tolerant and psychrotolerant bacterium,
designated NCCP-644T
, was isolated from a textile dyeing wastewater treatment pond. The optimum (and ranges of)
temperature and pH for growth of strain NCCP-644T was 20-25○C (3-37○C) and 7–8 (6-10), respectively. The strain can
tolerate 0-3% NaCl (w/v) and heavy metals (Cr 2700 ppm, As 3000 ppm, Pb 2100 ppm and Cu 2700 ppm) in tryptic soya
agar medium. The sequences of the 16S rRNA gene and three housekeeping genes, gyrB, rpoB and atpD, of strain NCCP644T
showed the highest similarity (98.3, 91.9, 95.3 and 96.6 %, respectively) with the strain "Acinetobacter kyonggiensis"
KSL5401-037T
. Phylogenetic analyses from the sequence of the16S rRNA, gyrB and rpoB genes also revealed the affiliation
of NCCP-644T with members of the genus Acinetobacter. The chemotaxonomic data [major quinones as Q-9; major cellular
fatty acids as summed feature 3 (iso-C15:0 2OH/C16:1ω7c) followed by C18:1 ω9c, C16:0, and C12:0 3-OH; major polar lipids as
diphosphatidyl glycerol, phosphatidyl serine, phosphatidyl monomethyl ethanol, phosphatidyl inositol, phosphatidyl
ethanolamine, phosphatidyl glycerol and one unknown phospholipid] also support the affiliation of strain NCCP-644T with
the genus Acinetobacter. The level of DNA–DNA relatedness between strain NCCP-644T
and the closely related type strains
of the genus Acinetobacter was < 54.7 %. Based on the phylogenetic analyses, DNA–DNA hybridisation and biochemical
characteristics, strain NCCP-644T
differed from the validly named Acinetobacter species and thus, it represents a new
species. The name, Acinetobacter pakistanensis sp. nov.is proposed for the type strain NCCP-644T
(= LMG 28467T
= KCTC
42081T
= JCM 18977T
).
