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Abstract
Drought is one of the major abiotic stresses to restrict cotton growth and productivity. Heat shock proteins are involved in cellular shielding of plant cells and tissues affected by dehydration. In this study, T2 segregating population of transgenic cotton containing small heat shock protein gene was compared with wild types under five and ten days drought stress. Seed germination was not affected significantly in both the lines. Amplification of 260bp GHSP26 gene through PCR and transgene integration was observed through Southern blot and fluorescence in-situ hybridization, confirmed the integration of 3 copies of transgene in the transgenic plants. Total leaf area and transpirational water loss in transgenic plants were significantly different from wild type. Ten days drought stress caused 58% ion outflow from wild type and only 40% from transgenic lines and biomass was also significantly reduced in wild type plants. Plants of both the lines were not affected for root shoot ratio under drought stress. Statistical analysis proved that chlorophyll content was maintained in transgenic plants, but it was reduced in wild type. These results showed that transgenic plants sustained growth under drought stress, so may be used for classical breeding to improve crops.

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Muhammad Bilal Sarwar, Fatima Batool, Bushra Rashid, Beenish Aftab,, Sameera Hassan, Tayyab Husnain. (2014) Integration And Expression Of Heat Shock Protein Gene In Segregating Population Of Transgenic Cotton For Drought Tolerance, , Volume-51, Issue-4.
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Pakistan Journal of Agricultural Sciences
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Volume-51
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