Abstract
In present exploration, purification and characterization of xylanase was carried out to find its optimum conditions for
maximum functionality. The xylanase (EC 3.2.1.8) synthesized by Aspergillus niger in submerged fermentation was partially
purified and characterized for different parameters like temperature, pH & heat stability. The molecular mass determined
through SDS-PAGE was found 30 kDa. The specific activity of the enzyme was raised from 41.85 to 613.13 with 48.63%
yield just in a two step partial purification comprising ammonium sulphate precipitation and Sephadex gel filteration column
chromatography. The partially purified enzyme was found to be optimally active at 60°C and 7.5 pH. Conclusively, for the
application of xylanase in food, feed or paper manufacturing processes, it is necessary to consider its optimum pH and
temperature.
