DNA markers are important tools for assessing genetic diversity and relationships among species, cultivars and breeding
materials. Many horticultural species are lacking genomic information. DNA markers that do not require prior knowledge of
DNA sequences are therefore appealing for horticultural research. A retrotransposon-based DNA marker system, iPBS (inter
primer binding sites) developed from conserved primer binding sites within retrotransposons, was used to study the genetic
variation and relationships in ornamental guava. PCR from 6 iPBS primers (dominant markers) produced a total of 113 bands
(52.38-100% polymorphic) ranging from 150 bp to 3000 bp, and the mean PIC value for each primer ranging from 0.1245 to
0.3698. Molecular information generated from both iPBS was separately scored in a matrix for phylogenetic dendrogram
construction. The phylogenetic dendrogram based on iPBS markers reflected morphologic classifications of the accessions
that were studied. The iPBS PCR-based genome fingerprinting technology in this study is low-cost and provides another
effective alternative in differentiation of accessions in guava (Psidium guajava Linn.) and related species or genera.
Asim Mehmood, Muhammad Jafar Jaskani, Saeed Ahmad, Rashid Ahmad. (2013) Evaluation Of Genetic Diversity In Open Pollinated Guava By iPBS Primers, , Volume-50, Issue-4.
