Abstract
Responses of the wheat genotype ‘Faisalabad-2008’ to plant regeneration using immature embryos and in vitro drought and salt stress tolerance were investigated. For this study immature embryos 15 DAP (Days After Pollination) were used and after 6 weeks healthy calli were transferred to media containing variable concentrations of NaCl (0, 5, 10, and 15g/L) and drought stress intensities using PEG 6000 (0, 5, 10, 15 and 20% (w/v)). The findings suggested that the embryogenic capacity of callus depended on concentrations of exogenous hormones and the genetic makeup of the genotype. Faisalabad-2008 showed good callus induction and ability to proliferate and regenerate seedlings under drought and salt stress conditions. This study will serve as a guide line in future for in vitro screening of wheat varieties for their regeneration ability using indirect methods. Further establishment of in-vitro screening protocols for abiotic stress tolerance will facilitate screening of genotypes during early developmental stages.

Sehar Nawaz, Nisar Ahmed, Ahsan Iqbal, Ihsan Khaliq. (2013) Optimization Of Regeneration Protocols For Wheat Under Drought And Salt Stress, , Volume-50, Issue-4.
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