Abstract
In this experiment, callus production and regeneration of Sainfoin (Onobrychis sativa var. Chadegan) were studied. The goal
of this research was to establish a new method for micro proliferation of Sainfoin. Optimization of the growth regulator
combinations in the Murashige and Skoog (MS) medium culture supplemented with combination of different 6-
benzylaminopurine (BAP) and 2,4-dichlorophenoxy acetic acid (2,4-D) concentrations was made. A large number of shoots
was propagated from four explants. The biggest callus (5.47cm) was obtained on the MS medium containing 2,4-D (6 mgl
-1
)
+ BAP (1 mgl
-1
) from apical meristem explant. The large number of shoots was produced in the culture medium
supplemented with BAP (4mg-1
) with average 1.6 plants per petri dish. The excised shoots were rooted in half strength MS
medium containing indole-3-butyric acid (IBA) (1 mg-1
) and transferred to perlite which grew into normal fertile plants. This
study showed that Sainfoin meristem explant, BAP and 2,4-D together were important for callogenesis and for regeneration
only BAP was effective.
